![]() ![]() Navigate Left Previous article in issue.These studies show that promoter elements responsible for unsaturated fatty acid-mediated transcription repression are tightly linked to OLE1 activation sequences and that OLE1 transcription levels are closely tied to acyl-CoA metabolism. Unsaturated fatty acid repression of OLE1 transcription, however, is not affected by the disrupted ACBP gene. Disruption of the ACBP gene causes a >5-fold activation of OLE1 transcription and a similar increase in OLE1 mRNA levels. Acyl-CoA binding protein (ACBP) plays a role in determining the level of FAR element activated transcription. ![]() Disruption of either gene results in reduced levels of unsaturated fatty acid repression disruption of both genes completely blocks the regulatory response. Two fatty acid activation genes, FAA1 and FAA4, were found to be essential for unsaturated fatty acid repression of OLE1 through the FAR sequences. The FAR element-mediated activation and fatty acid repression of transcription was found to be closely tied to fatty acyl-CoA metabolism. Essential elements required for unsaturated fatty acid repression of OLE1 were found in the 5′ and 3′ region of the 111-bp sequence. The 111-bp FAR element strongly activates transcription and confers unsaturated fatty acid regulation on a heterologous CYC1 promoter test plasmid. Deletion of an 88-bp sequence within that region resulted in a complete loss in transcription activation and unsaturated fatty acid regulation. ![]() A deletion analysis of OLE1 promoter:: lacZ fusion reporter genes identified a 111-base pair (bp) fatty acid-regulated (FAR) region approximately 580 bp upstream of the start codon that is essential for transcription activation and unsaturated fatty acid repression. Saturated fatty acids induce a 1.6-fold increase in transcription activity, whereas a large family of unsaturated fatty acids repress OLE1 transcription as much as 60-fold. ![]() One component of this regulation system involves the control of OLE1 transcription. OLE1 mRNA levels are differentially regulated by the addition of saturated or unsaturated fatty acids to the growth medium. In Saccharomyces cerevisiae, unsaturated fatty acids are formed from saturated acyl-CoA precursors by Ole1p, a Δ-9 fatty acid desaturase. ![]()
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The state of separation is so intolerable, so unachievable that it must obliterate itself. It cannot exist as a separate entity.Īny attempt to separate it is essentially the attempt to create a stable vacuum in nature. The self is other people, the world, nature, its environment. This is because the self is everything it experiences. When we try to separate ourself we create a state so empty, fragile, unstable and impossible that it has to immediately disappear. ‘That sounds a bit insulting Clare’ you might say ‘Are you calling me empty space? Are you saying I’m needy? Unstable? That I can’t exist?’ Let’s start with the fact that the separate human self is essentially a vacuum. ![]() Trying to separate out from everything creates a state so profoundly unstable that it immediately disappears.Īnd this has profound implications for us and what we want. Nothing cannot exist in nature because nature immediately fills that nothing with something. It is the attempt to create a nothingness within the all pervasive somethingness of the universe. The definition of a vacuum is true empty space – an environment with nothing in it. ![]() ![]() To better depict the character, he learned the Mengzi dialect, freediving, snorkeling, and deep diving. Xian's received his first starring role in the thriller detective web series Tientsin Mystic as the god river who is solves complicated case by his logical reason and judgement, Guo Deyou. Qin as one of the investigative team criminal police captain, Lin Tao. Also that year, he was cast in the first Chinese forensic web series Medical Examiner Dr. Later Xian was cast in the esports romance film Love O2O (2016), playing an online game developer team named Yu Banshan. He followed it with an appearance in the music video for the song I Am Jiang Xiaobai by Zhang Qi and the music video for the song Medals by Lu Han. These part led him to being cast in the feature film adapted from the web series. Xian then appeared in the coming-of-age brotherhood web series, Who Sleeps My Bro (2016) as a troubled university student named Xie Xun. He made his small-screen debut in the first China's urban web series City of Fantasy (2014), in which he played a depressed young widower who rely on drugs to continue to live after his wife passed away named A Liang. He followed it with a role in the romantic teen film Singing When We're Young (2012). Xian began his acting career with a minor role in the director Wang Jing's film Feng Shui (2012) as a young Li Xaoli. Career 2011–2018: Career beginnings and breakthrough For the convenience of entry, Xian changed his Chinese name from "晛" to "现", which means "present". ![]() So he retook the test and was enrolled in acting major at Beijing Film Academy in 2010. 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